DECODON - Delta2D: Image Fusion

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Image Fusion

Delta2D's image fusion lets you combine several gel images to one, new "ideal" gel. You can use Union fused images, containing all spots of all combined images, for making proteome maps, or Average fused images as a summary of groups of replicate gels.

The full text of the article that describes Delta2D's image fusion and its use for creating proteome maps is freely available online:

Luhn S, Berth M, Hecker M, Bernhardt J: Using standard positions and image fusion to create proteome maps from collections of two-dimensional gel electrophoresis images.
PROTEOMICS 2003 Jul;3(7):1117-27
Digital Object Identifier (DOI): 10.1002/pmic.200300433
PubMed Entry: PMID: 12872213

Related Features and Benefits

Combine images

Delta2D's proprietary image fusion lets you create proteome maps from collections of 2D gel images. A proteome map looks like a real gel but shows all spots on a single image - even those that will never be expressed at the same time. You can collect protein identifications on the map and then use Delta2D to automatically and reliably transfer them to new gels. Gel images can also be merged into an average image which combines replicates pixel-by-pixel. Read more about Proteome Maps.

Color spots according to their expression profiles.

The larger an experimental design, the higher the analytical complexity. Spot color coding condenses whole experiment expression profiles by using different colors in just one gel image. This simplifies extraction of the really interesting spots.

Standardized Spot Shapes due to Spot Transfer

Spot boundaries detected on union fusion images which contain the image information of a whole 2D gel study can be transfered to every gel of your experiment by using the Transfer Spots procedure of Delta2D. This results in uniform spot shapes over the whole experiment, generates complete expression profiles and prevents for the classical problems of multiple matches known from traditional 2D gel analyisis packages.



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