Report created by moser with Delta2D, Fri Jul 20 17:25:43 CEST 2007.
| Project Name | Demonstration | |
|---|---|---|
| Project Creation Date | Wed Feb 22 19:00:00 CET 2006 | |
| Author | Decodon | |
| Description | Delta2D demonstration project. A time-course experiment with three samples separated in duplicate. We thank Falko Hochgräfe for the gel images | |
| Use Internal Standard | no | |
| Pool | C:\Dokumente und Einstellungen\moser\.Delta2D\demopool |
| control (2 images) |
|---|
|
control_01
Control conditions. 983 x 1067 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots. |
|
control_02
Control conditions. 891 x 1015 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots. |
| 1 min (2 images) |
|
1min_01
1 minute after stimulus. 919 x 1063 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots. |
|
1min_02
1 minute after stimulus. 881 x 996 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots. |
| 10 min (2 images) |
|
10min_01
10 minutes after stimulus. 960 x 1060 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots. |
|
10min_02
10 minutes after stimulus. Flip Horizontal (07.05.2006) 932 x 1041 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots. |
| Fused Images (1 image) |
|
Fused Image
Fused Image showing all spots present on any of the gel images. This image is used as the basis for spot detection. Input: 10min_01, 10min_02, 1min_02, 1min_01, control_02, control_01 Function: Union Image Size: Common Region Warped to: control_01 922 x 1054 pixels / Typhoon9400 1_21025 1035 spots detected using algorithm 20060419 with parameters Local Background Region = 32, Average Spot Size = 10, Weak Spot Sensitivity = 20.0, Noise cutoff = 40.0, Use Spot Models = true. 67 cancelled spots. 29 labels |
| Sample | Image | Channel | Gel |
|---|---|---|---|
| A | 1min_01 | 35S met pulse | II |
| 1min_02 | 35S met pulse | V | |
| B | 10min_01 | 35S met pulse | III |
| 10min_02 | 35S met pulse | VI | |
| Control | control_01 | 35S met pulse | I |
| control_02 | 35S met pulse | IV | |
| Not assigned | Fused Image | Not assigned | Not assigned |
| control | |
|---|---|
control_01 |
control_02 |
| 1 min | |
1min_01 |
1min_02 |
| 10 min | |
10min_01 |
10min_02 |
| Fused Images | |
Fused Image |
|
| Master image | Warped (sample) image | Warp mode | Match vectors used |
|---|---|---|---|
| 10min_01 | 10min_02 | exact | 188 |
| 1min_01 | 1min_02 | exact | 95 |
| control_01 | 10min_01 | exact | 102 |
| control_01 | 1min_01 | exact | 115 |
| control_01 | Fused Image | global | 1 |
| control_01 | control_02 | exact | 210 |
10min_01 vs. 10min_02, exact warp, 188 match vectors |
1min_01 vs. 1min_02, exact warp, 95 match vectors |
control_01 vs. 10min_01, exact warp, 102 match vectors |
control_01 vs. 1min_01, exact warp, 115 match vectors |
control_01 vs. Fused Image, global warp, 1 match vector |
control_01 vs. control_02, exact warp, 210 match vectors |