Delta2D Project Report for Project 'Demonstration'

Report created by moser with Delta2D, Fri Jul 20 17:25:43 CEST 2007.

Project Name Demonstration
Project Creation Date Wed Feb 22 19:00:00 CET 2006
Author Decodon
Description Delta2D demonstration project. A time-course experiment with three samples separated in duplicate. We thank Falko Hochgräfe for the gel images
Use Internal Standard no
Pool C:\Dokumente und Einstellungen\moser\.Delta2D\demopool

Groups

control (2 images)
control_01
Control conditions.
983 x 1067 pixels / Typhoon9400 1_21025
1035 spots transferred from Fused Image
67 cancelled spots.
control_02
Control conditions.
891 x 1015 pixels / Typhoon9400 1_21025
1035 spots transferred from Fused Image
67 cancelled spots.
1 min (2 images)
1min_01
1 minute after stimulus.
919 x 1063 pixels / Typhoon9400 1_21025
1035 spots transferred from Fused Image
67 cancelled spots.
1min_02
1 minute after stimulus.
881 x 996 pixels / Typhoon9400 1_21025
1035 spots transferred from Fused Image
67 cancelled spots.
10 min (2 images)
10min_01
10 minutes after stimulus.
960 x 1060 pixels / Typhoon9400 1_21025
1035 spots transferred from Fused Image
67 cancelled spots.
10min_02
10 minutes after stimulus. Flip Horizontal (07.05.2006)
932 x 1041 pixels / Typhoon9400 1_21025
1035 spots transferred from Fused Image
67 cancelled spots.
Fused Images (1 image)
Fused Image
Fused Image showing all spots present on any of the gel images. This image is used as the basis for spot detection. Input: 10min_01, 10min_02, 1min_02, 1min_01, control_02, control_01 Function: Union Image Size: Common Region Warped to: control_01
922 x 1054 pixels / Typhoon9400 1_21025
1035 spots detected using algorithm 20060419 with parameters Local Background Region = 32, Average Spot Size = 10, Weak Spot Sensitivity = 20.0, Noise cutoff = 40.0, Use Spot Models = true.
67 cancelled spots.
29 labels

Samples

Sample Image Channel Gel
A 1min_01 35S met pulse II
1min_02 35S met pulse V
B 10min_01 35S met pulse III
10min_02 35S met pulse VI
Control control_01 35S met pulse I
control_02 35S met pulse IV
Not assigned Fused Image Not assigned Not assigned

Gel Images

control

control_01

control_02
1 min

1min_01

1min_02
10 min

10min_01

10min_02
Fused Images

Fused Image

Warping

Master image Warped (sample) image Warp mode Match vectors used
10min_01 10min_02 exact 188
1min_01 1min_02 exact 95
control_01 10min_01 exact 102
control_01 1min_01 exact 115
control_01 Fused Image global 1
control_01 control_02 exact 210

Dual Channel Images


10min_01 vs. 10min_02, exact warp, 188 match vectors

1min_01 vs. 1min_02, exact warp, 95 match vectors

control_01 vs. 10min_01, exact warp, 102 match vectors

control_01 vs. 1min_01, exact warp, 115 match vectors

control_01 vs. Fused Image, global warp, 1 match vector

control_01 vs. control_02, exact warp, 210 match vectors