About Delta2D
The Challenge
Delta2D: Dedicated to Innovation
Modern User Interface
How to use this manual
Contents
1
Strategy for Analyzing Gel Images with Delta2D
2
Install and start Delta2D
2.1
Available Computing Platforms
2.2
Installation Steps for Microsoft Windows
2.3
Start Delta2D
2.4
License Registration
2.5
Adjusting Memory Settings
2.6
Checking for Software Updates
2.7
Updating an Existing Installation of Delta2D
3
Workflow of Delta2D 4.0
3.1
Setup Project
3.2
Setup Gel Image Warping
3.3
Create Direct Warpings
3.4
Create the Consensus Spot Pattern
3.5
Analyze Expression Profiles
3.6
Present Results
3.7
Exporting Results to Other Applications
4
Main Menu and Toolbars
4.1
The Main Menu
4.2
The Main Toolbar
5
The Windows
5.1
Workflow
5.2
Project Explorer
5.3
Light Table
5.4
Warping Setup
5.5
Dual View
5.6
Quantitation Table
5.7
Gel Image Regions
5.8
Expression Profiles
5.9
Color Coding
5.10
Job Manager
5.11
Analysis
5.12
Project Matrix
5.13
Arrange Windows
6
The Gel Pool
6.1
Adding Gel Images to the Pool
6.2
Assigning Gel Image Attributes
7
Image Fusion
Why image fusion makes your work much more efficient
Image fusion algorithms
8
Working with Spots
8.1
Sorting and Selecting Spots
8.2
Selecting Spots in the Dual View
8.3
Hiding Spots
8.4
Canceling Spots
8.5
Marking Spots
8.6
Counting
8.7
Filtering
8.8
Scatter Plots
8.9
Spot Picking
9
Working with Spot Annotations
9.1
Creating a Label
9.2
Labels and Spots
9.3
Working with Labels
9.4
Formatting Labels
9.5
Creating and Using a Proteome Map with Spot Identifications
9.6
Support for Protein Identification by Mass Spectrometry
9.7
Scouts: Finding Information in Web Resources
10
Options
10.1
Delta2D
10.2
General
10.3
Memory
10.4
Keymap
11
Advanced Topic: Scripting
11.1
File Commands
11.2
Image Processing Commands
11.3
Commands for Controlling Windows
11.4
Remote Control Commands
12
Advanced Topic: Greyscale Calibration
13
Useful Tips
Create Your Own Pool Before You Start to Work with Delta2D
Adapt the Memory Settings of Delta2D
Working with Big Images
Accurate Scanning Will Be Recompensed
Working with Different Versions of Delta2D on the Same Data
Tuning Spot Detection
A
Example Files
B
Contact Information
C
Acknowledgments
D
Lists of Figures and Tables, Index
List of Figures
List of Tables