Documentation
The Delta2D Getting Started Guide shows how to analyze gel
images with Delta2D. You start with raw image data and finish with
quantitative results and visualizations that are ready for
presentation. This document is available in
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English:
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Deutsch:
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Français:
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Furthermore please refer to
Technical documentation
Application notes
- Differential
in gel electrophoresis (DIGE) - image analysis with Delta2D.
Delta2D supports this and other multiplex gel electrophoresis
techniques. It leverages advanced image processing algorithms like
image fusion to let you use internal standards, produce complete
expression profiles, and visualize the results - all in the same
package that you also use for the "classical" 2DE
experiments.
- 2D
Gel Scanning Guide. Getting sample preparation and conditions
for 2D gel electrophoresis right can take a lot of hard work and
patience. With all this effort, it is very easy to overlook the
importance of one essential step - scanning. Our 2D Gel Scanning
Guide contains practical tips that should be helpful for anyone
who cares about the quality of 2D gel image data.
Example Data
Download the Delta2D sample data for the example project described in the Getting Started guide or for a small multiplex experiment:
- Delta2D sample data ( 8.89 MB
) with the data for the example project:
six gels of Bacillus subtilis 168, treated with salt stress, in three groups of two replicates each:
- Control
- 1min (after 1 minutes)
- 10min (after 10 minutes)
Unpack the archive to some place on your computer, start Delta2D, and follow the guidelines of the
Getting Started Guide.
The sample data like gel images, match maps and quantitations can be found in the subfolder "samples" in the archive.
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Delta2D DIGE sample data
( 47.97 MB
) with the data for the DIGE example project:
three gels (Gel1, Gel2 and Gel3) containing three replicates of two samples each:
- Sample A (Cy3 images)
- Sample B (Cy5 images)
- Internal Standard (Cy2 images)
Looking for a software for 1D gel analysis?
If you are looking for an effective software package for the analysis of 1D electrophoresis gels, please visit the website of LabImage 1D, from our partner Kapelan Bio-Imaging.
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