Documentation

The Delta2D Getting Started Guide shows how to analyze gel images with Delta2D. You start with raw image data and finish with quantitative results and visualizations that are ready for presentation.

The Delta2D 4.1 Getting Started Guide is available in English for you to browse, or view as PDF. German version: Delta2D Erste Schritte (PDF).

Download the Delta2D sample data ( 8.89 MB ) for the example project described in the Getting Started guide. You can also use the Delta2D DIGE sample data if you prefer to try Delta2D with this kind of experiments.

Previous versions: Delta2D 3.6 Getting Started Guide English (PDF), Deutsch (PDF), Español (PDF), Chinese (PDF)

• What's New in Delta2D 4.1
• Delta2D product brochure

Technical documentation

• Delta2D manual, PDF Version browse online.
• Please refer to the Delta2D release notes if you are updating from a previous version.
• The statistical methods employed in Delta2D are described in the TMeV manual.

Application notes

• Differential in gel electrophoresis (DIGE) - image analysis with Delta2D. Delta2D supports this and other multiplex gel electrophoresis techiques. It leverages advanced image processing algorithms like image fusion to let you use internal standards, produce complete expression profiles, and visualize the results - all in the same package that you also use for the "classical" 2DE experiments.
• 2D Gel Scanning Guide. Getting sample preparation and conditions for 2D gel electrophoresis right can take a lot of hard work and patience. With all this effort, it is very easy to overlook the importance of one essential step - scanning. Our 2D Gel Scanning Guide contains practical tips that should be helpful for anyone who cares about the quality of 2D gel image data.

Example Data

• Delta2D sample data ( 8.89 MB ) with the data for the example project:
  six gels of Bacillus subtilis 168, treated with salt stress, in three groups of two replicates each:
  • Control
  • 1min (after 1 minutes)
  • 10min (after 10 minutes)
  Unpack to some place on your computer, start Delta2D, and when asked to open a project click on "Change Pool" and navigate to the example pool. The sample data like gel images, match maps and quantitations can be found in the subfolder "samples" within the pool folder.
• Delta2D DIGE sample data ( 47.97 MB ) with the data for the DIGE example project:
  three gels (Gel1, Gel2 and Gel3) containing three replicates of two samples each:
  • Sample A (Cy3 images)
  • Sample B (Cy5 images)
  • Internal Standard (Cy2 images)

Unpack the respective ZIP file to some place on your computer, start Delta2D, follow the guidelines of the Getting Started Guide and if necessary take the parts for DIGE experiments into account.

Looking for a software for 1D gel analysis?

If you are looking for an effective software package for the analysis of 1D electrophoresis gels, please visit the website of LabImage 1D, from our partner Kapelan Bio-Imaging.