Delta2D Report: Project Summary
for Project 'Demonstration'

Project Properties

Author		DECODON	Project Creation Date		Wed Feb 22 20:00:00 CET 2006
Use Internal Standard		no	Pool		C:\data\demopool
Description		Delta2D demonstration project. A time-course experiment with three samples. We thank Falko Hochgräfe for the gel images
Report created by user "bielz" with Delta2D, Fri Jul 15 11:46:11 CEST 2016. Please let us know if you have any comment or suggestion using our contact form.

Replicate Groups

Group: control (2 images)
control_01 Control conditions. 983 x 1067 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots 29 labels
control_02 Control conditions. 891 x 1015 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots

Group: 1 min (2 images)
1min_01 1 minute after stimulus. 919 x 1063 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots
1min_02 1 minute after stimulus. 881 x 996 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots

Group: 10 min (2 images)
10min_01 10 minutes after stimulus. 960 x 1060 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots
10min_02 10 minutes after stimulus. Flip Horizontal (07.05.2006) 932 x 1041 pixels / Typhoon9400 1_21025 1035 spots transferred from Fused Image 67 cancelled spots

Group: Fused Images (1 image)
Fused Image Fused Image showing all spots present on any of the gel images. This image is used as the basis for spot detection. Input: 10min_01, 10min_02, 1min_02, 1min_01, control_02, control_01 Function: Union Image Size: Common Region Warped to: control_01 922 x 1054 pixels / Typhoon9400 1_21025 1035 spots detected using algorithm 20060419 with parameters Local Background Region = 32, Average Spot Size = 10, Weak Spot Sensitivity = 20.0, Use Spot Models = true. 67 cancelled spots 29 labels

Samples and their Gel Images

Sample	Image	Channel	Gel
A		35S met pulse	II
A		35S met pulse	V
B		35S met pulse	III
B		35S met pulse	VI
Control		35S met pulse	I
Control		35S met pulse	IV
Not assigned		Not assigned	Not assigned

Warp pairs and their Dual Channel images

Master image	Warped (sample) image	Warp mode	Match vectors used
10min_01	10min_02		188
1min_01	1min_02		95
control_01	10min_01		102
control_01	1min_01		115
control_01	Fused Image		1
control_01	control_02		210