What does Delta2D do that I don't get with my present 2D gel analysis package?
Of course, that depends on what software you are currently using. Contact us for an in-depth answer. Here is a partial list of Delta2D's main benefits - most of them based on Delta2D's unique features:
Reliable results thanks to a unique analysis approach
In Delta2D, you can take advantage of a unique approach (warping – fusion – spot detection – boundary transfer) that leads to 100% spot matching with quantitation on the original images only. Besides higher throughput, this leads to significantly improved statistical confidence, so you can, for example, identify more biomarker candidates from the same experiment.
Intuitive user interface and workflow component
The recommended analysis approach for Delta2D is supported by a workflow component that leads you through the analysis. However, going back and forth is easy: You don't need to repeat each step you have already finished before.
Improved performance thanks to warping strategies and spot detection on one image only
With Delta2D, you'll only need a fraction of the time you spend with traditional packages. Warping compensates for running differences between gels. With Delta2D's warping strategies you can organize the warping according to your experimental design: Create your own or select one of the pre-defined strategies.
For instance, the Group Warping Strategy takes advantage of similarity of replicates. Thus, this strategy fastens automatic warping and simplifies manual quality control. Following Delta2D's recommend analysis approach, you create a fusion image for your project. The fusion contains all spots from all conditions. Thus, is a perfect image to detect a consensus spot pattern that can be used for all other images in the project.
Trust your results - apply advanced statistical methods easily
Delta2D offers many features (incl. views showing all images, customizable tables, dynamic expression profiles views, etc.) to check the quality of your results. Additionally, Delta2D incorporates the TIGR MeV tool which includes statistical methods such as t-Test, ANOVA, and non-paramatric tests like Wilcoxon or Kruskal-Wallis test to find differentially expressed spots. Many of these can be applied with false discovery rate (FDR) control. PCA, Hierarchical Clustering, and k-means/-medians clustering can be applied to find structures and identify outliers.
There are several options to export data from Delta2D. Besides basic features like image (e.g. as TIFF, PNG, etc.) and table exports (e.g. as csv file, to MS Excel, as pick lists), you are free to export data to MS Powerpoint (with spot boundaries and labels becoming real Powerpoint objects) or as interactive web reports in HTML file format.
For example, Delta2D's Proteome Maps give you a high-level view on a whole experiment. You can make maps that contain all identified proteins, color code them to show protein properties such as isoelectric point and molecular weight, highlight proteins with specific expression patterns, and more. Getting data out of Delta2D is easy — use the convenient exports to Excel, PowerPoint, and Text (CSV), or use the HTML based web reports to see your analysis results.
Easily connect to web ressources
Our unique Scout technology not only supports data retrieval from databases such as UniProt and GenoList, the technology can also be used to connect your in-house databases to Delta2D. Data is appended to your labels saved along with your project.
Visualize results in your preferred way
Delta2D offers a wide range of features for visualization. They help you to get your data ready for publication and presentation. Among these are color coding for spots (e.g. according to their intensities under certain conditions) and color coding for labels (e.g. according to pI).
Premium Support is our Standard
With Delta2D you'll not only get an analysis software. Licenses include support: Our experts support you during your first steps with Delta2D... and even further.
All in one package and for all plattforms
If you decide for Delta2D, you don't need to purchase any additional modules, add-ons or something similiar. Delta2D allows you to analyze all kinds of 2D gel experiments such as classic stainings (e.g. Coomassie, Sypro Ruby, etc.), multiplexing (e.g. DIGE or Refraction-2D), phospho- or glycoproteomics, comparisons of 2D western blots with gels, and many more. Delta2D is based on Java. Therefore, you can use it on Windows, Mac or Linux based systems.
Last update on 2012-09-03 by Detlev Bielz.