Quantitative Analysis of 2DE Gels: Based on Warping, Spot Detection, and Normalization
You are analyzing 2D gels and are wondering why "image warping" is such a helpful technology? You don't know which warping strategy should be used?
You like to use optimal settings for spot detection? You are uncertain about background subtraction or spot normalization and would like to check and – if necessary – correct it?
DECODON Academy Webinar
Quantitative Analysis of 2DE Gels:
Image Warping, Spot Detection, and Normalization
answers these questions by focusing on:
- Which Warping Strategy should be chosen?
- How can I find optimal parameters for spot detection?
- When and how do I to split, add, and merge spots to get reliable quantities?
- Which normalization method shall I choose and what do I have to take into consideration?