The Getting Started Guide helps to get through an example analysis as quick as possible - following our recommended workflow. It is available for normal as well as for DIGE experiments. Read it online, print it, or ask us for a copy - and enjoy working with Delta2D!
Each version of the Getting Started Guide is referring to a complete set of example data. Please find each data set below.
The Getting Started Guide is available in different languages.
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2D Western Blot experiments
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Download the Delta2D sample data for the example projects described in the Getting Started guides or for a small multiplex experiment, unpack the archive to some place on your computer, start Delta2D, and follow the guidelines of the Getting Started Guide. The sample data like gel images, match maps and quantitations can be found in the subfolder "samples" in the archive.
Delta2D sample data (8.89 MB) with the data for the normal example project:
six gels of Bacillus subtilis 168, treated with salt stress, in three groups of two replicates each:
- 1min (after 1 minutes)
- 10min (after 10 minutes)
We thank Falko Hochgräfe for the gel images.
Delta2D DIGE sample data (47.97 MB) with the data for the DIGE example project:
three gels (Gel1, Gel2 and Gel3) containing three replicates of two samples each:
- Sample A (Cy3 images)
- Sample B (Cy5 images)
- Internal Standard (Cy2 images)
We thank Dr. Maria Zellner and Prof. Dr. Rudolf Oehler (Medical University of Vienna) for the DIGE gel images.
Delta2D WesternBlot sample data (9.1 MB) with the data for the WesternBlot example project:
five images including Western Blot and Contour images, in detail:
- Total Protein Gel
- Total Protein Membrane
- Total Protein Membrane Contour
- Western Blot 1
- Western Blot Contour
Sample: human mesothelial cell line
Total protein detection: UV light (stain free approach)
1st antibody: O-GlcNAc
2nd antibody: anti mouse Ig/HRP
Antibody detection: ECL (chemiluminescence)
We thank PD Dr. Ing. Klaus Kratochwill and his team from the Medical University of Vienna for the images.